Silica Gel 60A Suggested Use Link SOP
Silica Gel 60A
Standard Operating Procedure (SOP)
Silica Gel 60A is ideal for normal-phase chromatography solvent mixtures and DIY column
chromatography. It’s also recommended for in-line hydrocarbon CRC applications, using a
small plug of Silica Gel 60A at 1–10% m/m of the estimated extract in solution.
Safety: Please adhere to the safety and handling
instructions in the Silica Gel 60A Safety Data Sheet (SDS), included with your shipment, available at
mediabros.store, or by emailing sales@mediabros.store.
Required Materials:
- Silica Gel 60A Filtration Media
-
In-line Filtration Housing & Filter
- CRC Column Size: For best results, use a 4–6 inch diameter column.
- Filter: Optimized for use with a ≤5-micron screen filter followed by a 0.2-micron, Media Bros Ulti-Filter.
Recommended Conditions:
Solvent-to-biomass ratio: 6:1 to 10:1
Operating temperature: Below -10°C, will not clog due to ice
Flow rate: < 1 GPM. A needle valve or flow restrictor at the column base
improves flow control.
Procedure:
-
Determine your ratio. For every pound of biomass, load 150–250 grams
of Silica Gel 60A into the filtration housing. The exact amount depends on biomass quality and desired
outcome. Refer to the quick reference table:
Silica Gel 60A (Media Use Based on Grade)
Plant Matter Low Grade Medium Grade High Grade 1 lb 250g 200g 150g 2 lbs 500g 400g 300g 5 lbs 1,250g 1,000g 750g 10 lbs 2,500g 2,000g 1,500g 15 lbs 3,750g 3,000g 2,250g 20 lbs 5,000g 4,000g 3,000g 25 lbs 6,250g 5,000g 3,750g 30 lbs 7,500g 6,000g 4,500g 50 lbs 12,500g 10,000g 7,500g 100 lbs 25,000g 20,000g 15,000g - Secure filter screen and media. Ensure the filter screen is safely installed. A cloth or paper filter may be combined with a screen or plate. Load media directly into the column on top of the filter screen.
- PRE-WETTING REQUIRED FOR USE WITH CHROMATOGRAPHY APPLICATIONS. Silica Gel 60A is designed to be used dry except for use in chromatography applications.
- RUN EXTRACTION USING A HYDROCARBON-BASED SOLVENT OR CHROMATOGRAPHY MOBILE PHASE SOLVENT COMBINATION. Avoid saturation and maintain flow. Any lost yield can be recovered by flushing the media with pure solvent.
- Disposal. Follow all SDS instructions for safe disposal.
